Thermo Scientific™ Phire Plant Direct PCR Master Mix

Amplify DNA directly from plant samples without prior DNA purification with Phire Plant Direct PCR Master Mix

Supplier:  Thermo Scientific™ F160L

Catalog No. F160L

Description

Thermo Scientific™ Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR. The kit is based on Phire Hot Start II DNA Polymerase, a specially engineered enzyme with a DNA binding domain. The unique features of this DNA polymerase make it extremely robust and tolerant of many PCR inhibitors present in plant material like complex polysaccharides, polyphenols and others. Phire Plant Direct PCR Master Mix has been tested with leaves and seeds from a wide variety of plant species. The kit includes a complete set of optimized reagents, detailed protocols making it an ideal choice for amplification of plant DNA. When combined with Thermo Scientific™ Arktik™ Thermal Cycler, PCR protocols can be completed in as little as 40 minutes.

Highlights

• Direct PCR - sample is added directly to PCR reaction, therefore there is no need for time-consuming and expensive DNA purification steps.
• Dilution & Storage protocol available - allows tens of PCR reactions from one tiny sample and allows re-testing
• Specially engineered Phire Hot Start II DNA polymerase - extremely short PCR protocol times
• Master Mix format with premixed gel loading dye - minimizes possibility of cross-contamination, reduces sample handling and allows direct loading on gel

Applications

• Genotyping
• Transgene detection
• Knockout analysis
• Sequencing

Includes

• 2x Phire Plant Direct PCR Master Mix
• Dilution Buffer
• Control Primer Mix (25 µM each)
• Water, nuclease- free
• O'GeneRuler Express DNA Ladder

Specifications

• Product Type: Direct PCR Master Mix
• Product Line: Phire
• Polymerase: Phire Hot Start II DNA polymerase
• GC-Rich PCR Performance: Medium
• Overhang: Blunt
• Content And Storage: Contains:
  • 10 x 1.25 mL Phire Plant Direct PCR Master Mix (2X)
  • 2 x 5 mL Dilution Buffer
  • 40 µL Control Primer Mix (25 µM each)
  • 10 x 1.25 mL Water, nuclease- free
  • 50 µg O'GeneRuler Express DNA Ladder (sufficient for 100 applications)
  
  Store at -20°C.

• For Use With (Application): Direct PCR
• Hot Start: Built-In Hot Start
• Reaction Speed: Fast
• Reaction Format: SuperMix or Master Mix
• No. of Reactions: 1250 x 50 L Reactions
• Quantity: 1250 x 50μL rxns