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Invitrogen™ Permeabilization Medium (Medium B)
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Permeabilization Medium (Medium B)

Supplier:  Invitrogen™ GAS002S100

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Catalog No. GAS002S100


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Description

Description

Permeabilization Medium (Medium B) is a component of the FIX & PERM™ Cell Permeabilization Kit. The kit is used in flow cytometry for fixing and permeabilizing cells in suspension, allowing access of antibody to intracellular targets without sacrificing scatter characteristics.

The FIX & PERM™ Cell Permeabilization Kit is:

  • Flexible—compatible with multiple sample types and cellular antigens
  • Robust—scatter pattern of cells unaffected
  • Efficient—simultaneous detection of intracellular and surface markers

    View a selection guide for all available FIX & PERM™ products and products related to sample preparation (including cell lysis and preservation solutions, blocking reagents, and information about the use of Dynabeads™ cell separation products for cell isolation).

    The Permeabilization Medium formula was specifically designed to reduce background staining and allow simultaneous addition of fluorochrome-labeled antibodies during the permeabilization step.

    How It Works
    Cells are incubated for 15 minutes at room temperature with Fixation Medium (Medium A) (Cat. No. GAS001S100) and washed. Cells are then incubated with the Permeabilization Medium and fluorochrome-labeled antibody. Both Fixation Medium and Permeabilization Medium are included in the FIX & PERM™ Cell Permeabilization Kit.
    • Specifications

    Specifications

    Room Temperature
    FIX & PERM™
    Permeabilization Reagent
    Permeabilization Medium (Medium B)
    1 Each
    Room Temperature
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    For Research Use Only. Not for use in diagnostic procedures.