Gibco™ PeproGMP® Human LIF Recombinant Protein, PeproTech®

Recombinant Protein

Leukemia inhibitory factor (LIF) is a 20 kDa protein that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190 or LIFR, and the subunit gp130, which is shared with the other members of the IL-6 family. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissue. LIF can be up-regulated by pro-inflammatory cytokines such as TNFα and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal.Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse-specific.

Accession Number

P15018

For Use With (Application)

Functional Assay

Format

Lyophilized

Formulation

Protein with no preservative

Molecular Weight (g/mol)

19.7

Name

LIF

Quantity

100 μg

Immunogen

Human LIF

Storage Requirements

-20°C or -80°C if preferred

Regulatory Status

RUO

Endotoxin Concentration

<0.1

Assays

Functional Assay

Conjugate

Unconjugated

Recombinant

Recombinant

Activity

Determined by the proliferation of TF-1 cells.

Expression System

E. coli

Product Line

PeproGMP

Purity or Quality Grade

Purified