Invitrogen™ NuPAGE™ 4 to 12%, Bis-Tris, 1.0–1.5 mm, Mini Protein Gels

Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels that provide broad molecular weight protein separation with high resolution and sample integrity.

Catalog Number	Mfr. No.	Wells	Gel Thickness	Quantity
NP0321BOX	Invitrogen™ NP0321BOX	10-well		10 Gels/Box
NP0321PK2	Invitrogen™ NP0321PK2	10-well	1.0 mm	2 Gels/Box
NP0323BOX	Invitrogen™ NP0323BOX	15-well		10 Gels/Box
NP0323PK2	Invitrogen™ NP0323PK2	15-well	1.0 mm	2 Gels/Box
NP0324BOX	Invitrogen™ NP0324BOX	1-well		10 Gels/Box
NP0327BOX	Invitrogen™ NP0327BOX	9-well	1.0 mm	10 Gels/Box
NP0329BOX	Invitrogen™ NP0329BOX	17-well	1.0 mm	10 Gels/Box
NP0329PK2	Invitrogen™ NP0329PK2	17-well	1.0 mm	2 Gels/Box
NP0330BOX	Invitrogen™ NP0330BOX	IPG-well		10 Gels/Box
NP0335BOX	Invitrogen™ NP0335BOX	10-well	1.5 mm	10 Gels/Box
NP0335PK2	Invitrogen™ NP0335PK2	10-well	1.5 mm	2 Gels/Box
NP0336BOX	Invitrogen™ NP0336BOX	15-well		10 Gels/Box
NP0336PK2	Invitrogen™ NP0336PK2	15-well	1.5 mm	2 Gels/Box

Description

Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels that provide broad molecular weight protein separation with high resolution and sample integrity. These precast gels are ideal for applications where protein integrity is crucial. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is important.

Features of NuPAGE Bis-Tris gels:
• Better protein integrity—optimized sample preparation process preserves your proteins
• Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
• Faster run times—get separation of your proteins in as little as 35 minutes
• Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperature

Choose the right NuPAGE Bis-Tris gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.

NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer (NP0007) and NuPAGE Sample Reducing Agent (NP0004). Use NuPAGE Antioxidant (NP0005) in the running buffer to maintain the reduced state of the proteins during the run and to allow maximum band sharpness. The gels can be run using NuPAGE MES SDS Running Buffer (NP0002) to better resolve small proteins or NuPAGE MOPS SDS Running Buffer (NP000102) to resolve medium- to large-size proteins.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer (NP00061) for traditional wet transfer using the Mini Blot Module (B1000) or XCell II Blot Module (EI9051). Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device (IB21001).

Specifications

• For Use With (Equipment): Mini Gel Tank, XCell SureLock Mini-Cell
• Gel Size: Mini
• Separation Range: 3.5 to 260 kDa
• Mode of Separation: Molecular Weight
• Storage Requirements: Store at 4–25°C. Do not freeze.
• Width (Metric): 8 cm

• Gel Percentage: 4 to 12%
• Gel Type: Bis-Tris
• Separation Type: Denaturing
• Shipping Condition: Room Temperature
• Length (Metric): 8 cm

Safety and Handling

ShelfLife : 12 Months