MilliporeSigma™ Novagen™ Overnight Express™ Autoinduction NMR Medium-¹⁵N

Provides reagents to make a chemically defined autoinducing liquid culture medium used to produce [U-¹⁵N] labeled proteins for NMR spectroscopy. Novagen™ Overnight Express™ Autoinduction NMR Medium- ¹⁵N system includes four concentrated sterile solutions.

Supplier:  MilliporeSigma™ 717594

Catalog No. 71-759-4

Description

• Autoinduction method is based on the differential metabolism of carbon sources to promote growth to high cell density
• Automatically induce protein expression under control of lac promoters, eliminating the need to monitor cell densities or add inducer (IPTG)
• Includes four concentrated sterile solutions:
• OnEx NMR Solution 1 is a blend of carbon sources optimized for tightly regulated uninduced growth to high density, followed by autoinduction
• OnEx NMR Solution 2 is a buffer and nitrogen blend that mediates metabolic acid production and provides additional nitrogen for increased protein synthesis
• OnEx Solution 3 provides magnesium for maximal cell density
• OnEx Solution 4 provides trace metals to minimize growth limitations associated with mineral deficiencies and satisfy the requirements of metal-containing target proteins, even at high expression levels

Specifications

• Quantity: 5 L Kit
• Content And Storage: Multiple storage requirements. Ok to freeze.
• Product Type: NMR Medium-¹⁵N