Cytiva Ni Sepharose™ 6 Fast Flow

Combines the advantages of Ni2 for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform. Cytiva Lifescience™ Ni Sepharose™ 6 Fast Flow is part of a range of IMAC chromatography media for capture and intermediate purification.

Catalog Number	Mfr. No.	Quantity
45-002-987	Cytiva 17531806	5 mL
45-002-984	Cytiva 17531801	25 mL
45-002-985	Cytiva 17531802	100 mL
45-002-986	Cytiva 17531803	500 mL

Description

Ni Sepharose 6 Fast Flow is a member of the Cytiva range of IMAC chromatography media for capture and intermediate purification.

• Designed for scaling up purification of histidine-tagged proteins.
• High protein binding capacity and minimal leakage of Ni2+ ions.
• As a BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
• Available in convenient prepacked HisPrep and HisTrap column formats.
• Composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2+ ions for purification of histidine tagged proteins.
• The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2+ leakage while providing high capacity.
• Available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
• As member of the BioProcess media range, Ni Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Specifications

• Buffer: 0.1M HCl, 0.1M NaOH, 8M Urea
• Height (English): 9.84 in.
• Flow Rate: 600 cm/hr.
• pH Range: 3 to 12
• Support Type: 6% Cross-linked Agarose
• Matrix: Ni-Sepharose

• Product Type: Ni Sepharose 6 FF chromatography media
• Height (Metric): 250mm
• Max. Pressure: 1 bar
• Particle Size: 90μm
• Content And Storage: 4° to 30°C