Invitrogen™ Neon™ Transfection System 100 μL Kit

Neon™ Transfection System 100 μL Kit

Supplier:  Invitrogen™ MPK10096

Catalog No. MPK10096

Includes: 30mL Resuspension Buffer R, 30mL Resuspension Buffer T, 2 × 150mL Electrolytic Buffer E2, 96 × 100μL Neon™ Tips, 20 Neon Electroporation Tubes

Description

• For use with the Neon Transfection System
• Use this kit for transfection volumes of 100μL, containing 5 x 10⁵-2 x 10⁶ adherent cells or 1 x 10⁶-5 x 10⁶ suspension cells
• Cells that have been transfected using the included 100μL Neon Tips are then ready to be washed and plated into a 6-well, 60mm, or 10cm culture dish, depending on protocol

Adenoviral shRNA, Cell Culture, Lentiviral shRNA & miRNA, Plasmid Transfection, RNAi, RNAi Transfection, RNAi, Epigenetics & Noncoding RNA Research, Retroviral RNAi, Stem Cell & Primary Cell Transfections, Synthetic siRNA Transfection, Transfection, Vector-Based RNAi, siRNA

Order Info

Shipping Condition: Room Temperature

Specifications

• Concentration: 5 x 10^5 - 2 x 10^6 cells/mL (Adherent Cells), 1 x 10^6 - 5 x 10^6 cells/mL
• Quantity: 96 x 2 Reactions
• System Type: Neon™ Transfection System
• Serum Compatible: Yes
• Shipping Condition: Room Temperature
• No. of Reactions: 96 x 2
• For Use With (Equipment): Neon™ Transfection System
• Classification: Animal Origin-Free, Chemically Defined, Low-Protein, Xeno-Free, Reduced Growth Factors, Reduced Serum
• For Use With (Application): Transfection

• Cell Type: Stem Cell, Primary Cell, Hard-To-Transfect Cell
• Format: 6-Well Plate, 12-Well Plate, 24-Well Plate, 48-Well Plate, 96-Well Plate, Flasks
• Volume (Metric) Sample: 100µl
• Starting Material Amount: 2x10^4 - 6 x10^8 cells; Varies by cell type
• Product Type: Transfection System Kit
• Product Line: Neon™
• Sample Type: Plasmid DNA, Synthetic siRNA
• Content And Storage: • 30 mL Resuspension Buffer R
  • 30 mL Resuspension Buffer T
  • 2 x 150 mL Electrolytic Buffer E2
  • 96 x 100 µL Neon™ Tips
  • 20 Neon™ Electroporation Tubes
  
  Store the buffer box 4°C and the tips & tubes box at room temperature.
• Transfection Technique: Electroporation