Invitrogen™ Neon™ Transfection System 100 μL Kit

Neon™ Transfection System 100 μL Kit

Supplier:  Invitrogen™ MPK10025

Catalog No. MPK10025

Description

The Neon™ Transfection System 100 μL Kit is designed specifically for use with the MPK5000. Use this kit for transfection volumes of 100 μL, containing 5 × 10⁵–2 × 10⁶ adherent cells or 1 × 10⁶–5 × 10⁶ suspension cells. Cells that have been transfected using the included 100μL Neon™ Tips are then ready to be washed and plated into a 6-well, 60 mm, or 10 cm culture dish, depending on your protocol.

Specifications

• Product Type: Transfection System
• Quantity: 25 x 2 reactions
• Promoter: CMV
• Classification: Animal Origin-Free, Chemically Defined, Low-Protein, Xeno-Free, Reduced Growth Factors, Reduced Serum
• Sample Loading Volume: 100µl
• Shipping Condition: Room Temperature
• System Type: Neon™ Transfection System
• For Use With (Equipment): Neon™ Transfection System
• Transfection Technique: Electroporation

• For Use With (Application): Transfection
• Format: Electroporation Pipette Tip
• Content And Storage: The Neon™ Transfection System 100 µL Kit comes with 10 mL resuspension buffer R, 10 mL resuspension buffer T, and 75 mL E2 electrolytic buffer in one box and 25 x 100µL reaction tips and 5 electroporation tubes in a separate box. Refer to the Neon™ Transfection System users manual for protocol instructions. Store the buffer box at 4°C and the tips & tubes box at room temperature.
• For Use With: Transfection Grade Nucleic Acid purification kits, siRNA vectors, DNA vectors
• Serum Compatible: Yes
• Starting Material Cell No.: 2x10^4 - 6 x10^8 cells; Varies by cell type
• Product Line: Neon™
• Sample Type: Plasmid DNA, Synthetic siRNA
• Cell Type: Established Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells