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New England Biolabs, Inc. NEBuilder HiFi DNA Assembly Cloning Kit – 10 reactions
Supplier: New England Biolabs, Inc. E5520S
NEBuilder HiFi DNA Assembly Master Mix was developed to improve the efficiency and accuracy of DNA assembly. This method allows for seamless assembly of multiple DNA fragments, regardless of fragment length or end compatibility. This method has been used to assemble either single-stranded oligonucleotides or different sizes of DNA fragments with varied overlaps (15-30 bp). It has utility for the synthetic biology community, as well as those interested in one-step cloning of multiple fragments due to its ease of use, flexibility and simple master-mix format. The cloning kit contains NEB 5-alpha Competent Cells.
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