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New England Biolabs, Inc. NEB PCR Cloning Kit (Without Competent Cells) – 20 reactions
Supplier: New England Biolabs, Inc. E1203S
This PCR Cloning Kit contains an optimized Cloning Mix containing a proprietary ligation enhancer and a linearized vector that uses a novel mechanism for background colony suppression to give a low background. It allows simple and quick cloning of any PCR amplicon, whether the amplification reactions are performed with proofreading DNA polymerases, such as Q5 which produce blunt ends; or nonproofreading DNA polymerases, such as Taq or Taq mixes (OneTaq, LongAmp Taq) which produce single base overhangs. Possible due to invisible end polishing components in the master mix that are active during the ligation step only if needed. Kit also allows direct cloning from amplification reactions without purification, and works well whether or not the primers used in the PCR possess 5'-phosphate groups. The ultimate in flexibility: clone with any amplicon made with any DNA polymerase, with or without 5' phosphates, purified or not!
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