N,N'-Bis(acryloyl)cystamine, Electrophoresis Reagent, 98%, Thermo Scientific Chemicals

Description

BAC is used as a reversible cross-linker for polyacrylamide gels, to simplify isolation of macromolecules separated on the gels. The disulfide linkage can be broken with a suitable reducing agent. A 1-g slice of a 4% (12:1) gel will dissolve in 5 mL of 100 mM DTT in TBE (pH 8.3) in less than 30 min. The pH of 8.3 is optimum for rapid dissolution of the gel.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
BAC is used as a reversible cross-linker for polyacrylamide gels, to simplify isolation of macromolecules separated on the gels. The disulfide linkage can be broken with a suitable reducing agent. A 1-g slice of a 4% (12:1) gel will dissolve in 5 mL of 100 mM DTT in TBE (pH 8.3) in less than 30 min. The pH of 8.3 is optimum for rapid dissolution of the gel.

Solubility
Soluble at 50 mg/ml in acetic acid

Notes
Store at -20°C. Incompatible with heat, oxidizing agents.

Specifications

Chemical Identifiers

• CAS: 60984-57-8
• Molecular Formula: C10H16N2O2S2
• Molecular Weight (g/mol): 260.37
• MDL Number: MFCD00036225
• InChI Key: DJVKJGIZQFBFGS-UHFFFAOYSA-N
• Synonym: n,n'-bis acryloyl cystamine, n,n'-bisacrylylcystamine, 2-propenamide, n,n'-dithiodi-2,1-ethanediyl bis, 2,2'-bisacrylamino diethyldisulfide, bis-acrylylcystamine: bac, n,n'-dithiodi-2,1-ethanediyl bis acrylamide, n-2-2-prop-2-enamido ethyl disulfanyl ethyl prop-2-enamide, n-2-2-prop-2-enoylamino ethyldisulfanyl ethyl prop-2-enamide, acmc-20ak9p, n,n-bis-acrylylcystamine
• PubChem CID: 100602
• IUPAC Name: N-[2-[2-(prop-2-enoylamino)ethyldisulfanyl]ethyl]prop-2-enamide
• SMILES: C=CC(=O)NCCSSCCNC(=O)C=C

• Melting Point: 123°C to 124°C
• Color: White
• Quantity: 5 g
• Solubility Information: Soluble at 50mg/mL in acetic acid
• Formula Weight: 260.37
• Percent Purity: 98%
• Physical Form: Powder
• Chemical Name or Material: N,N'-Bis(acryloyl)cystamine, Electrophoresis Reagent

Safety and Handling

Hazard Category	H315-H319-H335
Hazard Statement	GHS H Statement H315-H319-H335 Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.
Precautionary Statement	P261-P264b-P271-P280-P302+P352-P304+P340-P305+P351+P338-P312-P332+P313-P362-P501c
EINECSNumber	262-546-8
TSCA	No
storageNote1	−30°C to -10°C
Recommended Storage	Store at -20°C; Store under Nitrogen

SDS

RUO – Research Use Only