Mouse anti-Rat IgG1 Secondary Antibody, eFluor™ 615, eBioscience™, Invitrogen™

Mouse Monoclonal Secondary Antibody

Supplier:  Invitrogen 42481282

Catalog No. 50-112-9216

Description

Description: The R1-12D10 monoclonal antibody recognizes rat IgG1 antibodies and can be used as a second step reagent in microscopy. The monoclonal does not recognize other rat isotypes nor does it crossreact to mouse IgG1. Applications Reported: This R1-12D10 antibody has been reported for use in microscopy, immunohistochemical staining, and immunocytochemistry. Applications Tested: This R1-12D10 antibody has been tested by immunohistochemistry on frozen mouse tissue and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filter Recommendation: When using this eFluor™ 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor™ 594 filter is acceptable. Excitation: 595 nm; Emission: 615 nm. Filtration: 0.2 μm post-manufacturing filtered.

Secreted immunoglobulins (Ig) have two separable functions: one is to bind specifically to pathogenic molecules, the other is to recruit cells and molecules to destroy the opsonized pathogen.

Specifications

• Antigen: Rat IgG1
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Target Species: Rat
• Form: Liquid

• Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
• Clone: R1-12D10
• Conjugate: eFluor 615
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Secondary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Isotype: IgG

For Research Use Only.