Invitrogen™ Red/Green BODIPY™ PC-A2 (1-O-(6-BODIPY™ 558/568-Aminohexyl)-2-BODIPY™ FL C5-Sn-Glycero-3-Phosphocholine) (Ratiometric Phospholipase A2 Substrate)

Measure results in microplate assays or using flow cytometry

Supplier:  Invitrogen™ A10072

Catalog No. A10072

Description

Molecular Probes phospholipase substrates are dye labeled phospholipids used to monitor phospholipase A (PLA) activity in purified enzyme preparations, cell lysates, and living cells. Two structural variations of these substrates are available, which confer specificity for PLA1 and PLA2. Fluorescently labeled versions of the cleavage product generated from these substrates are also available for use as standards for evaluating percentage substrate conversion in enzymatic reactions.

• Label (Ex/Em): Red/Green BODIPY™ PC-A2 (approx. 488/530,590nm) (can be used in dual emission fluorescence ratio detection)
• Specificity: PLA₂
• Cleavage product: BODIPY FL C5
• Lyophilized product can be dissolved in DMSO for use
• Fluorescence is measured either in microplate assays or using flow cytometry

Enzyme and Protein Activity Assays, Lipase and Phopholipase Activity, Proteins, Expression, Isolation and Analysis

Order Info

Shipping Condition: Room temperature

Specifications

• Cell Permeability: Cell-Permeant
• Detection Method: Fluorescence
• Product Line: BODIPY™
• Substrate: Phospholipase A2 substrate (ratiometric)
• Target Enzyme: Phospholipase
• Shipping Condition: Room Temperature

• Content And Storage: Store in freezer (-5 to -30°C).
• Form: Solid
• Quantity: 100 μg
• Substrate Properties: Lipid-Based Substrate
• For Use With (Equipment): Flow Cytometer, Microplate Reader

For Research Use Only. Not for use in diagnostic procedures.