Thermo Scientific™ Melon™ Gel Monoclonal IgG Purification Kit

Purify mouse monoclonal antibodies from serum, ascites or hybridoma cell culture supernatant with this high-yield, mild monoclonal IgG purification kit.

Supplier:  Thermo Scientific™ 45214

Catalog No. PI45214

Description

Thermo Scientific™ Melon Gel Monoclonal IgG Purification Kit is a high-yield, mild purification system for mouse monoclonal antibodies from serum, ascites or hybridoma cell culture supernatant.

The Melon Gel Monoclonal IgG Purification Kit consists of a large package size of Melon Gel Resin and an optimized protocol for easy and mild purification of monoclonal IgG antibodies from hybridoma culture supernatant or ascites fluid. Unlike typical bind-and-elute affinity methods, Melon Gel Purification is based on the binding of non-IgG proteins from a prepared supernatant or ascites sample, thereby allowing the pure IgG to be recovered in the flow-through (non-bound) fraction. Antibodies are recovered ready for use in assays or subsequent conjugation and other modification steps. There is no need to neutralize or desalt to mitigate the effects of harsh elution buffers. Melon Gel Purification saves time compared to classical Protein A or Protein G methods without compromising the quality of the antibody.

Highlights:

• Fast – purify antibodies in half the time of required for Protein A or G

• Efficient – recovery and purity is often better than Protein A or G purification

• Robust – effective for subclasses of IgG that bind poorly to Protein A or G

• Gentle – antibodies retain activity because they are not exposed to denaturing conditions

• Reusable – support can be regenerated and used for up to 6 purifications

• Compatible – amine-free buffer does not require removal or neutralization

• Flexible – accommodates a broad range of sample volumes and processing vessels

Includes:

Melon Gel Resin, purification buffer and resin regenerant

Specifications

• Product Type: IgG Purification Kit
• Column Type: Proprietary Resin, Affinity
• Stationary Phase: Proprietary Ligand
• Description: Melon Gel Monoclonal IgG Purification Kit
• Quantity: 200 mL

• Product Line: Melon™
• Format: Kit, Bottle
• Purification Target: IgG Antibodies
• Content And Storage: Sufficient For: IgG purification from up to 1 L of cell culture supernatant or up to 200 mL of ascites fluid
  • Melon Gel Support, 200 mL
  • Melon Gel Purification Buffer (dry mix to make 100X), 1 L
  • Melon Gel Regenerant (dry mix to make 5X), 1 L
  
  Store at 4°C

For Research Use Only. Not for use in diagnostic procedures.