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Thermo Scientific™ Melon™ Gel IgG Spin Purification Kit
Rapidly purify human, mouse and rabbit IgG from serum and other sources with this simpler alternative to traditional Protein A and Protein G methods.
Supplier: Thermo Scientific™ 45206
Description
Thermo Scientific™ Melon Gel IgG Purification Resins and Kits comprise a simpler and faster alternative to traditional Protein A and Protein G methods for purification of human, mouse and rabbit IgG from serum and other sources.
Melon Gel IgG Purification Kits enable fast, easy and gentle purification of antibodies from serum and other sample types. Antibody purification with Melon Gel may be performed with gravity-flow columns, spin columns or chromatography cartridges. Melon Gel IgG Purification saves time compared to classical Protein A or Protein G methods without compromising the quality of the antibody. Compared to antibody affinity purification with Protein A or Protein G, Melon Gel IgG Purification is simpler, faster and provides greater yields and higher purity for a wider variety of antibodies species.Highlights:
Simple, one-step protocol – IgG Purification with Melon Gel Resin does not require the binding, washing, and multiple elution steps required by Protein A and Protein G
Rapid purification – antibody purification from serum is four to six times faster than Protein A or G methods
High recovery and purity – antibody recovery from serum for many species is greater than 90% with greater than 80% purity
Minimal dilution – reduced need to concentrate antibody before storage or use
Robust purification – Melon Gel IgG Purification Kits work with a wide range of antibodies including many that do not purify well on Protein A or Protein G
Gentle purification – no harsh antibody elution conditions helps retain IgG activity
Reusable support – Melon Gel Resin can be used for multiple antibody purifications
Available in various formats – spin columns, purification kits and chromatography cartridges
Specifications
IgG Purification Kit | |
Proprietary Resin, Affinity | |
Proprietary Ligand | |
Melon™ Gel IgG Spin Purification Kit, 3mL, Spin Column, Kit, Microcentrifuge Format, Affinity, Proprietary Resin Column Type, Proprietary Ligand Immobilized Phase, Sufficient For IgG Purification From Approximately 25 x 0.1mL Serum Samples | |
3 mL |
Melon™ | |
Kit, Microcentrifuge Spin Column | |
IgG Antibodies | |
Sufficient For: IgG purification from approx. 25 x 0.1 mL serum samples (total approx. 25 mg IgG from serum) • Melon Gel Support, 3 mL • Melon Gel Purification Buffer, 100 mL • Spin Columns, 0.8 mL, 27 columns • Microcentrifuge Tubes, 2 mL, 30 tubes Store at 4°C |
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For Research Use Only. Not for use in diagnostic procedures.