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Gibco™ Medium 199 (2X)
SDP

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells.

Supplier:  Gibco™ 21157029

Catalog No. 21-157-029


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Description

Description

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco™ Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This Medium 199 is modified as follows:
With: L-glutamine
Without: HEPES, Phenol Red
The complete formulation is available.

Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This Medium 199 is made with Earle's salts.

Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Specifications

Specifications

Storage conditions: 2 – 8°C. Protect from light.
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
2 X
Liquid
Sterile-filtered
No HEPES, No Phenol Red
cGMP-compliant under the ISO 13485 standard
250 mL
Mammalian Cells
Mammalian Cell Culture
Medium 199
Glutamine, Earle's Balanced Salt Solution (EBSS)
Sustainable packaging
Gibco™
Room Temperature
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Safety and Handling

ShelfLife : 12 Months

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For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.