Abnova™ MDM2/CEN12 CISH Probe

MDM2/CEN12 CISH Probe is designed for the qualitative detection of human MDM2 gene and chromosome 12 alpha satellites in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).

Supplier:  Abnova™ CG0009

Catalog No. 89-335-550

Description

The probe is intended to be used in combination with the CISH Implementation Kit 2, which provides necessary reagents for specimen pretreatment and post-hybridization processing.

Hybridization signals of digoxigenin-labeled polynucleotides appear dark green distinct dot-shaped (MDM2 gene region), and dinitrophenyl-labeled polynucleotides appear bright red colored distinct dots (CEN 12).
Normal situation: In interphases of normal cells or cells without a amplification involving the MDM2 gene locus, two green signals and two red signals appear.
Aberrant situation: In cells with amplification of the MDM2 gene locus, an increased number of gene specific signals or signal clusters are visible. Other signal distribution may be observed in some abnormal samples which might result in a different signal pattern than described above, indicating variant rearrangements.
Unexpected signal patterns should be further investigated.

Specifications

• Content And Storage: The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18 to 25°C) and mix briefly before use.
• For Use With (Application): CISH-P