Invitrogen™ LanthaScreen™ TR-FRET PPAR alpha Competitive Binding Assay Kit, goat

LanthaScreen™ TR-FRET PPAR alpha Competitive Binding Assay Kit, goat

Supplier:  Invitrogen™ PV4892

Catalog No. PV4892

Description

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15143:

The LanthaScreen™ TR-FRET PPAR alpha Competitive Binding Assay provides a sensitive and robust method for high-throughput screening (HTS) of ligands for peroxisome proliferator-activated receptor alpha (PPAR alpha). The kit uses a terbium-labeled anti-GST antibody, a fluorescent small-molecule pan-PPAR ligand (Fluormone™ Pan-PPAR Green), and a human PPAR alpha ligand-binding domain (LBD) that is tagged with glutathione S-transferase (GST), in a homogeneous mix-and-read assay format.

To assay:
When running the LanthaScreen™ TR-FRET PPAR alpha Competitive Binding Assay, Fluormone™ Pan-PPAR Green is added to ligand test compounds followed by addition of a mixture of the PPAR alpha-LBD and terbium anti-GST antibody. When the Fluormone™ Pan-PPAR Green is bound to PPAR alpha, energy transfer from the terbium-labeled antibody to the tracer occurs, and a high TR-FRET ratio is observed. Competitive ligand binding to PPAR alpha is detected by a test compound’s ability to displace the tracer, which results in a loss of FRET between the antibody and the tracer. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the IC50 from a dose response curve of the compound (Figure 1). This type of binding assay is analogous to radioligandbased assays, except that it eliminates handling of radioactivity and enables a homogeneous addition-only format.

Specifications

• Conjugate: Tb (Terbium)
• For Use With (Equipment): Microplate Reader
• Content And Storage: The LanthaScreen™ TR-FRET PPAR alpha Competitive Binding Assay Kit contains PPAR alpha-LBD (GST) protein, Fluormone™ Pan-PPAR Green, terbium-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).
• Readout: End Point
• Target Entry: PPARA, PPAR alpha, NR1C1
• For Use With (Application): Competitive Binding Assay, TR-FRET
• Product Type: TR-FRET PPAR Alpha Competitive Binding Assay Kit

• Detection Method: Fluorescent
• Packaging: 384-well Plate
• Assay Entry: Biochemical competitive binding
• Shipping Condition: Dry Ice
• Product Line: LanthaScreen™
• Ligand: Pan-PPAR

For Research Use Only. Not for use in diagnostic procedures.