Invitrogen™ LanthaScreen™ TR-FRET FXR Coactivator Assay Kit, goat

LanthaScreen™ TR-FRET FXR Coactivator Assay Kit, goat

Supplier:  Invitrogen™ PV4833

Catalog No. PV4833

Description

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15140:

The LanthaScreen™ TR-FRET FXR (farnesoid X receptor) Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential FXR ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein- labeled coactivator peptide, and an FXR ligand-binding domain (FXR-LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous, mix-and-read assay format.

To assay:
When running the LanthaScreen™ TR-FRET FXR coactivator assay in agonist mode, FXR-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and used to determine the EC50 from a dose response curve of the compound. Based on the biology of the FXR-coactivator peptide interaction, the ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).

Specifications

• Conjugate: Tb (Terbium)
• For Use With (Equipment): Microplate Reader
• Content And Storage: The LanthaScreen™ TR-FRET FXR Coactivator Assay Kit contains FXR-LBD (GST) protein, fluorescently labeled SRC2-2 coactivator peptide, Tb-labeled anti-GST antibody, and buffers.
  
  Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).
• Gene ID (Entrez): 9971
• Shipping Condition: Dry Ice
• Product Line: LanthaScreen™
• Ligand: FXR

• Detection Method: Fluorescent
• Packaging: 384-well Plate
• Assay Entry: Biochemical coregulator interaction
• Readout: End Point
• Target Entry: FXR, NR1H4
• For Use With (Application): Co-Factor Interaction Assay, TR-FRET
• Product Type: TR-FRET FXR Coactivator Assay Kit

For Research Use Only. Not for use in diagnostic procedures.