Invitrogen™ T7 RNA Polymerase

DNA-dependent RNA polymerase with high specificity for bacteriophage T7 promoter sequences

Supplier:  Invitrogen™ 18033100

Catalog No. 18-033-100

Includes: T7 RNA Polymerase is supplied with a vial of 5X reaction buffer [200mM Tris-HCl (pH 8.0), 40mM MgCl₂ , 10mM spermidine-(HCl)₃ , 125mM NaCl], vial of 100mM DTT.

Description

Synthesizes large quantities of RNA from DNA inserted into a transcription vector downstream from a T7 promoter. A T7 RNA Polymerase technical bulletin is available.

• Application: Synthesis of labeled and unlabeled RNA transcripts.
• Source: Purified from E. coli expressing the T7 RNA Polymerase gene on a plasmid
• Performance and Quality Testing: 3' and 5' exodeoxyribonuclease, ribonuclease, and DNA nicking assays; performance in a transcription reaction
• Unit Definition: One unit hydrolyzes 1nMol of ribonucleotide into acid-precipitable material in 1 hr at 37°C using a T7 transcription vector as template
• Unit Reaction Conditions: 40mM Tris-HCl (pH 8.0), 25mM NaCl, 8mM MgCl₂, 2mM spermidine-(HCl)₃, 5mM DTT, 0.4mM ATP, 0.4mM CTP, 0.4mM GTP, 0.4mM UTP, 1μCi [^{3H]GTP, 1μg SphI-cut pT7L13, and enzyme in 50μL for 10 min. at 37°C}

Cell Free Protein Expression for Functional Analysis, Cell Free Protein Expression for Structural Analysis, Cell-Free (in vitro) Expression, Gene Expression Analysis and Genotyping, In Vitro Transcription, Protein Expression, Proteins, Expression, Isolation and Analysis

Order Info

Shipping Condition: Wet Ice

Specifications

• Content And Storage: T7 RNA Polymerase (50 U/μL) is supplied with a vial of 5X reaction buffer [200 mM Tris-HCl (pH 8.0), 40 mM MgCl₂ , 10 mM spermidine-(HCl)₃ , 125 mM NaCl], vial of 100 mM DTT. Store at -20°C.
• Polymerase: T7 RNA Polymerase
• Promoter: T7
• Quantity: 2 x 2500 U

For Research Use Only. Not for use in diagnostic procedures.