Invitrogen™ SuperScript™ III First-Strand Synthesis SuperMix

The SuperScript III First-Strand Synthesis SuperMix is an optimized formulation for first-strand cDNA synthesis from purified poly(A)⁺ or total RNA. RNA targets from 100 bp to >12 kb can be detected with this system.

Supplier:  Invitrogen™ 18080400

Catalog No. 18-080-400

Includes: 2X Reaction Mix (500μL); SuperScript III Enzyme Mix (100μL); Oligo(dT)₂₀ (50μL total provided at 50μM); Random hexamers (50μL total provided at 50ng/μL); Annealing buffer (50μL)

Description

The SuperScript III First-Strand Synthesis SuperMix is an optimized formulation for first-strand cDNA synthesis from purified poly(A)⁺ or total RNA. RNA targets from 100 bp to >12 kb can be detected with this system. The amount of starting material can vary from 0.1 pg to 5 μg of total RNA.

This kit includes SuperScript III/RNaseOUT enzyme mix, 2X First-Strand reaction mix, and an annealing buffer. SuperScript III Reverse Transcriptase is a version of MMLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme can be used to synthesize cDNA at a temperature range of 45–55°C, providing increased specificity, higher yields of cDNA, and more full-length product than many previous reverse transcriptases.

Using SuperScript III First-Strand Synthesis SuperMix
SuperScript III RT is not significantly inhibited by ribosomal and transfer RNA and can be used to synthesize cDNA from total RNA. RNaseOUT Recombinant RNase Inhibitor is included in the enzyme mix to safeguard against degradation of target RNA due to ribonuclease contamination. The 2X First-Strand reaction mix includes 10 mM MgCl₂ and 1 mM of each dNTP in a buffer formulation that has been optimized for first-strand synthesis of cDNA. The annealing buffer is used in the initial template-primer annealing step. Separate tubes of oligo(dT)₂₀ and random hexamers are also provided. cDNA synthesis can be performed using either total RNA or poly(A)⁺-selected RNA primed with oligo(dT), random primers, or a gene-specific primer.

Order Info

Shipping Condition: Dry ice

Specifications

• Content And Storage:

  • 2X Reaction Mix, 500 μL
  • SuperScript™ III Enzyme Mix, 100 μL
  • Oligo(dT)₂₀, 50 μL total (50 μM)
  • Random hexamers, 50 μL (50 ng/μL)
  • Annealing buffer, 50 μL
  
  Store at –20°C.
  
  

• GC-Rich PCR Performance: High
• Technique: Reverse Transcription
• Primer: Random Primers, Oligo dT Primers
• Shipping Condition: Dry Ice
• Final Product Type: First-Strand cDNA
• Reaction Format: Master Mix
• Starting Material: RNA

• Format: Kit
• Reaction Speed: 50 min.
• Optimal Reaction Temperature: 50°C
• Reverse Transcriptase: SuperScript III
• For Use With (Application): Real Time PCR (qPCR)
• No. of Reactions: 50 Reactions
• Reagent Type: Reverse Transcription

For Research Use Only. Not for use in diagnostic procedures.