Invitrogen™ Platinum™ Taq DNA Polymerase

Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable 'hot start' thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase.

Includes: Each box contains: Platinum Taq DNA Polymerase; 10X PCR Buffer (no magnesium chloride); 50mM magnesium chloride; KB Extender

Catalog Number	Mfr. No.	No. of Reactions
10-966-018	Invitrogen™ 10966018	120 Reactions
10-966-026	Invitrogen™ 10966026	300 Reactions
10-966-034	Invitrogen™ 10966034	600 Reactions
10-966-083	Invitrogen™ 10966083	5000 Reactions

Description

Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable hot-start thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase. The hot start property of the enzyme is conferred by thermolabile monoclonal antibodies that render Taq DNA polymerase inactive until the initial PCR denaturation step, thus preventing the extention of nonspecifically annealed primers and improving product yield.

The hot-start property allows for convenient reaction assembly at room temperature. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a non-template dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends and has a 5' to 3' exonuclease activity. PCR products generated with Platinum Taq DNA Polymerase may be used in the same downstream applications without protocol modifications.

Features

• Convenient reaction assembly at room temperature
• 5' to 3' exonuclease activity
• Lacks 3' to 5' exonuclease activity

Applications

• Amplification of DNA from complex genomic, viral, and plasmid templates
• Hot-start PCR
• RT-PCR

Notes

• For superior PCR performance, the next-generation enzyme Platinum II Taq Hot-start DNA Polymerase is recommended.
• Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology.

Order Info

Shipping Condition: Approved for shipment on wet or dry ice

Specifications

• Format: Tube
• Polymerase: Platinum Taq DNA Polymerase
• Product Type: DNA Polymerase
• Fidelity (vs. Taq): 1X
• Overhang: 3'-A
• Size (Final Product): 5 kb or less

• GC-Rich PCR Performance: Low
• Exonuclease Activity: 5' - 3'
• For Use With (Application): Hot-start PCR
• Hot Start: Built-In Hot Start
• Reaction Format: Separate Components
• Starting Material: DNA

For Research Use Only. Not for use in diagnostic procedures.