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Invitrogen™ pBAD/gIII Kit
Express recombinant proteins under the tight regulation of the araBAD promoter
Supplier: Invitrogen™ V45001
Includes: T20μg each of pBAD/gIII A, B and C, 20μg of pBAD/gIII/calmodulin, 1mL 20% L-arabinose, LMG194 and TOP10 E. coli stabs.
Description
- To simplify purification, vector pBAD/gIII encodes a leader peptide which directs recombinant protein into the periplasmic space
- Features araBAD promoter for tightly regulated expression
- Translation initiation signals for optimized E. coliexpression
- Leader peptide from bacteriophage fd gene III protein (gIII) for secreted expression
- C-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin
- C-terminal c-myc epitope for detection and analysis with an Anti-myc Antibody
- A set of three vectors is provided (A, B, and C): Each has the C-terminal tag in a different reading frame relative to the multiple cloning site to simplify in-frame cloning of the gene
Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
Specifications
Inducible | |
araBAD | |
None | |
Ampicillin (AmpR) | |
Restriction Enzyme/MCS |
Arabinose | |
Bacterial Expression Vector | |
The pBAD/gIII Kit includes 20 µg each of pBAD/gIII A, B, & C, 20 µg of pBAD/gIII/calmodulin, 1 ml 20% L-arabinose, LMG194 and TOP10 E. coli stabs. Store stabs at 2 - 8°C. Store all other components at -20°C. All components are guaranteed stable for 6 months when properly stored. | |
c-Myc Epitope Tag | |
pBAD |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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