Invitrogen™ Taq DNA Polymerase, recombinant

Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.

Supplier:  Invitrogen™ 10342053

Catalog No. 10-342-053

Includes: Taq DNA Polymerase (5U/μL); 10X PCR buffer (200mM Tris-HCl pH 8.4, 500mM KCl); Magnesium Chloride (50mM)

Description

Taq DNA Polymerase, recombinant, is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. This recombinant enzyme is purified from the cloned Thermus aquaticus DNA polymerase gene expressed in E. coli and consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5' to 3' DNA polymerase activity and a 5' to 3' exonuclease activity.

Features

• A choice of recombinant or native enzyme
• Amplification of PCR products up to 5 kb in size
• Enzyme is licensed and qualified for PCR

Applications

• Amplification of DNA from complex genomic, viral, and plasmid templates
• RT-PCR
• Sequencing ssDNA
• Cycle sequencing

Notes

• One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 minutes at 74°C.
• For superior PCR performance, DreamTaq DNA Polymerase is recommended.

Order Info

Shipping Condition: Approved for shipment on wet or dry ice

Specifications

• Content And Storage: • Taq DNA Polymerase (5 U/μL), 20 μL
  • 10X PCR buffer, 1.5 mL
  • 50 mM MgCl₂, 1 mL
  
  Store at -10°C to -30°C in a non-frost-free freezer.
  Guaranteed stable for 6 months when stored properly.
• GC-Rich PCR Performance: Low
• Polymerase: Taq DNA Polymerase
• Exonuclease Activity: 5' - 3'
• Quantity: 100 Units
• For Use With (Application): Standard PCR
• Fidelity (vs. Taq): 1X
• No. of Reactions: 100 Reactions
• Reaction Format: Separate Components
• Starting Material: DNA

• Format: Stand-alone enzyme
• PCR Method: qPCR, Standard PCR
• Reaction Speed: Standard
• Product Type: DNA Polymerase
• Shipping Condition: Dry Ice
• Concentration: 5 U/μL
• Hot Start: No
• Overhang: 3'-A
• Size (Final Product): 5 kb or less

For Research Use Only. Not for use in diagnostic procedures.