Invitrogen™ Dynabeads™ Mouse CD43 (Untouched™ B Cells)

Use this kit to isolate pure and viable untouched B cells from mouse lymphoid organs by negative isolation. The kit depletes T cells, monocytes/macrophages, NK cells, plasma cells, CD5+ B1 cells, platelets, dendritic cells, granulocytes and erythrocytes.

Supplier:  Invitrogen™ 11422D

Catalog No. 11-422-D

Includes: 5mL MyOne; Dynabeads- coated with streptavidin and biotinylated rat monoclonal CD43 antibody against mouse T cells, monocytes/macrophages, NK cells, plasma cells, CD5+ B1 cells, platelets, dendritic cells, granulocytes and erythrocytes

Description

Use this kit to isolate pure and viable untouched B cells from mouse lymphoid organs by negative isolation. The kit depletes T cells, monocytes/macrophages, NK cells, plasma cells, CD5+ B1 cells, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated mouse B cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free B cells are truly untouched, and in perfect shape for any functional assay and application.

High purity, recovery and viability of mouse untouched B cells
• Easy to use and to scale up
• No columns required

Methodology
Dynabeads Mouse CD43 (Untouched B Cells) is added directly to the sample and binds the unwanted cells. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched mouse B cells can be directly analyzed in a flow cytometer and used in any application.

Starting Sample
Spleen or lymph nodes

Order Info

Shipping Condition: Room Temperature

Specifications

• Cell Type: B Cells
• Product Type: Antibody Coated Bead
• Format: Bead
• No. of Cells: Processes ∼2 x 10⁹ cells/kit, Processes ∼2 x 10⁹ cells total, Processes ∼1 x 10⁹ cells/kit
• Product Line: DYNAL™, Dynabeads™, Untouched™
• Quantity: 1 kit
• Sample Type: Lymph Node, Tissue Digests, Lymph nodes, Spleen
• Starting Material Cell No.: 5 x 10⁷ cells/test

• Content And Storage: The Kit Contains: 5 mL MyOne™ Dynabeads™-coated with streptavidin and biotinylated rat monoclonal CD43 antibodyagainst mouse T cells, monocytes/macrophages, NK cells, plasma cells, CD5+ B1 cells, platelets, dendritic cells, granulocytes and erythrocytes.
  
  Store at 2°C to 8°C.
• Final Product Type: Cells
• Isolation Technology: Negative Isolation
• No. of Tests: 40
• Purity or Quality Grade: Research Grade
• Reactivity: Mouse
• Shipping Condition: Room Temperature
• Target Species: Mouse

For Research Use Only. Not for use in diagnostic procedures.