Invitrogen™ Dynabeads™ Mouse Pan T (Thy1.2)

Dynabeads™ Mouse pan T (Thy 1.2) are magnetic beads that provide a robust and versatile tool to isolate or deplete mouse T-cells directly from any sample.

Supplier:  Invitrogen™ 11443D

Catalog No. 11-443-D

Includes: 5mL Dynabeads Mouse pan T coated with Thy1.2 monoclonal antibody. Thy1.2 antigen is expressed on mouse peripheral T cells, thymocytes and intra-epithelial T lymphocytes of the most commonly used mouse strains

Description

Dynabeads™ Mouse pan T (Thy 1.2) are magnetic beads that provide a robust and versatile tool to isolate or deplete mouse T-cells directly from any sample, including tissue digests such as spleen or lymph nodes, whole blood, bone marrow, and mononuclear cells (MNC).
• Rapid isolation of mouse T-cells from any sample—no columns required
• Positive isolation for molecular downstream assays or efficient depletion of mouse T cells

Excellent Yield, Purity, and Viability of Recovered Cells
Dynabeads™ Mouse pan T (Thy 1.2) are uniform, superparamagnetic beads (4.5 μm diameter) that enable easy isolation of mouse T-cells from spleen or lymph nodes, although any single-cell suspension may be used. Dynabeads™ Mouse pan T beads are coated with a primary monoclonal antibody specific for the Thy 1.2 membrane antigen expressed on peripheral T cells, thymocytes and intraepithelial T lymphocytes of all common mouse strains. Dynabeads™ Mouse pan T binds to T-cells during a short incubation period in a tube, after which the bead-bound cells are separated from unbound cells using a magnet. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity, recovery and viability of the isolated T cells.

Ideal for Both Depletion and Positive Isolation for Downstream Molecular Assays
Because of the powerful magnets used for separation, Dynabeads™ Mouse pan T (Thy 1.2) can be used in viscous samples such as single cell suspensions from tissue, whole blood and bone marrow, and enable efficient depletion of mouse T-cells in about 30 minutes. Dynabeads™ Mouse pan T will also afford excellent recovery of high-purity, viable cells when used for positive isolation for downstream studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note intact cells will not be released from these beads, so if you want to isolate mouse T-cells for cell-based applications, or you need to check your samples with flow cytometry, you should use Dynabeads™ FlowComp Mouse Pan T (CD90.2) (Cat. No. 11465D) (for bead-free cells).

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

Order Info

Shipping Condition: Room Temperature

Specifications

• Cell Type: T Cells (Whole Population)
• Product Type: Antibody Coated Bead
• No. of Cells: Processes ∼2 x 10⁹ cells total
• Product Line: DYNAL™, Dynabeads™
• Quantity: 5 mL
• Sample Type: Lymph Node, Blood, Spleen
• Starting Material Cell No.: 1 x 10⁷ cells per isolation
• Diameter (Metric): 4.5 μm

• Content And Storage: This Product Contains: 5 mL Dynabeads™ Mouse pan T coated with Thy1.2 monoclonal antibody. Thy1.2 antigen is expressed on mouse peripheral T cells, thymocytes and intra-epithelial T lymphocytes of the most commonly used mouse strains.
  
  Store at 2°C to 8°C.
• Isolation Technology: Depletion, positive isolation for molecular applications
• Output Viability: >95%
• Purity or Quality Grade: Research Grade
• Reactivity: Mouse
• Shipping Condition: Room Temperature
• Target Species: Mouse

For Research Use Only. Not for use in diagnostic procedures.