Invitrogen™ Dynabeads™ FlowComp™ Mouse CD8 Kit

This gentle and easy-to-use kit allows you to isolate flow-compatible mouse CD8+ T cells. Immediately after positive isolation, the beads are released and removed from your cell sample.

Supplier:  Invitrogen™ 11462D

Catalog No. 11-462-D

Includes: 1mL Antibody Mix, 3mL FlowComp; Dynabeads and 40mL FlowComp; Release Buffer

Description

This gentle and easy-to-use kit allows you to isolate flow-compatible mouse CD8+ T cells. Immediately after positive isolation, the beads are released and removed from your cell sample. In this way, you avoid exposing your cells to potentially cytotoxic or immunogenic foreign substances like iron oxides or dextrans, a potential problem when working with biodegradable particles. In addition, your cells will not be exposed to the stress of being passed through a column.

You can expect a purity level of >85% and viability of >85% of mouse CD8+ cells.

Advantages
• No risk of your results being affected by the isolation method
• Gentle tube-based method—no columns needed
• High purity, recovery and viability
• Bead-free cells—compatible with flow cytometry analysis
• Viable, bead-free CD8+ T-cells for direct use in any cell-based assay.

The isolated cells have the presumed profile of memory and naive mouse CD8+ T cells, and show a high proliferation capacity. You will save time and money, and get increased confidence in your results.

Starting sample
Spleen or lymph node cells

Order Info

Shipping Condition: Room Temperature

Specifications

• Cell Type: T Cells (CD8)
• Product Type: Superparamagnetic Beads
• No. of Cells: Processes ∼2 x 10⁹ cells total
• Product Line: DYNAL™, Dynabeads™, FlowComp™
• Quantity: 1 kit
• Sample Type: Lymph Node, Blood, Spleen
• Starting Material Cell No.: 5 x 10⁷ cells per isolation

• Content And Storage: The Kit Contains:
  • 1 mL Antibody Mix
  • 3 mL FlowComp™ Dynabeads™
  • 40 mL FlowComp™ Release Buffer.
  
  Store at: 2°C to 8°C.
• Isolation Technology: Positive Isolation
• Output Viability: >95%
• Purity or Quality Grade: Research Grade
• Reactivity: Mouse
• Shipping Condition: Room Temperature
• Target Species: Mouse

For Research Use Only. Not for use in diagnostic procedures.