Invitrogen™ Dynabeads™ CD15

Dynabeads™ CD15 are superparamagnetic beads covalently coupled with an anti-human CD15 antibody that efficiently depletes human CD15+ myeloid cells (predominantly neutrophil and eosinophil granulocytes) directly from whole blood, buffy coat, or PBMC samples.

Supplier:  Invitrogen™ 11137D

Catalog No. 11-137-D

Includes: 5mL Dynabeads CD15 coated with anti-CD15 monoclonal antibody.

Description

Dynabeads™ CD15 are superparamagnetic beads covalently coupled with an anti-human CD15 antibody that efficiently depletes human CD15+ myeloid cells (predominantly neutrophil and eosinophil granulocytes) directly from whole blood, buffy coat, or PBMC samples. Dynabeads™ CD15 can also be used to positively isolate CD15+ cells for molecular applications. Advantages of Dynabeads™ CD15:

• Efficient depletion of granulocytes (neutrophils and eosinophils)
• Isolation of granulocytes directly from whole blood for molecular applications
• No columns required

About Dynabeads™ CD15
Dynabeads™ CD15 are uniform, superparamagnetic polystyrene beads (4.5 μm diameter) coated with a monoclonal mouse IgM antibody recognizing the human CD15 membrane antigen. The CD15 antigen is predominantly expressed on human neutrophil and eosinophil granulocytes, and to a varying degree on monocytes.

Magnetic bead-;based separation offers easy handling
Dynabeads™ CD15 is added to the sample under continuous mixing to optimize the binding of the Dynabeads™ to the target cells. By placing the sample on a magnet, it separates the bead-bound target cells from the rest of the sample in just 1–2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, PBMC, or buffy coat.

Order Info

Shipping Condition: Room Temperature

Specifications

• Cell Type: Granulocytes
• Material: Polystyrene
• Isolation Technology: Depletion, positive isolation for molecular applications
• Output Viability: >95%
• Purity or Quality Grade: Research Grade
• Shipping Condition: Room Temperature
• Target Species: Human

• Content And Storage: This Product Contains: 5 mL Dynabeads™ CD15 coated with anti-CD15 monoclonal antibody.
  
  Storage Conditions: 2°C to 8°C. Do not freeze.
• Product Type: Antibody Coated Bead
• No. of Cells: Processes ∼2 x 10⁹ cells total
• Product Line: DYNAL™, Dynabeads™
• Sample Type: PBMC, Blood
• Starting Material Cell No.: 1 x 10⁷ PBMCs per isolation
• Diameter (Metric): 4.5 μm

For Research Use Only. Not for use in diagnostic procedures.