Invitrogen™ DNA Polymerase I, Large (Klenow) Fragment

DNA polymerase enzyme that lacks the 5' to 3' exonuclease activity of intact DNA Polymerase I, but does exhibit the 5' to 3' DNA polymerase and 3' to 5' exonuclease activities

Includes: DNA Polymerase I, Large (Klenow) Fragment is supplied with a vial of 10X REact™ 2 Buffer [500mM Tris-HCl (pH 8.0), 100mM MgCl₂, 500mM NaCl], vial of dilution buffer.

Catalog Number	Mfr. No.	Quantity
18-012-021	Invitrogen™ 18012021	100 Units
18-012-039	Invitrogen™ 18012039	500 Units

Description

Applications:
Fill-in of 5' overhangs . Synthesis of probes by random primers labeling method. Sequencing single- and double-stranded DNA. Site-directed mutagenesis.

Source:
Purified from E. coli expressing the Klenow fragment on a plasmid.

Performance and Quality Testing:
SDS-PAGE purity; single-stranded and double-stranded endodeoxyribonuclease and self-priming assays; performance evaluated in fill-in reaction.

Unit Definition:
One unit incorporates 10nMol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a template•primer.

Array CGH, Cloning, Gene Expression Analysis and Genotyping, Genotyping and Genomic Profiling, Restriction Enzyme Cloning

Order Info

Shipping Condition: Approved for shipment on Wet or Dry Ice

Specifications

• Content And Storage: DNA Polymerase I, Large (Klenow) Fragment is supplied with a vial of 10X REact™ 2 Buffer [500 mM Tris-HCl (pH 8.0), 100 mM
  MgCl₂ , 500 mM NaCl], vial of dilution buffer. Store at -20°C.
• Polymerase: DNA Polymerase I
• Hot Start: No

For Research Use Only. Not for use in diagnostic procedures.