Invitrogen™ DNA Polymerase I/DNase I

An optimized mixture of both enzymes for efficient nick translation of DNA

Supplier:  Invitrogen™ 18162016

Catalog No. 18-162-016

Includes: DNA Polymerase I/DNase I contains 0.5 units DNA Polymerase I and 0.4 milliunits DNase I per microliter.

Description

• Application: Labeling DNA with either radiolabeled or biotinylated nucleotides
• Source: DNase I is purified from bovine pancreas; DNA Polymerase I from E. coli λ lysogen nM 964
• Performance and Quality Testing: Performance tested in nick translation reaction
• Unit Definition: One unit of DNA Polymerase I in the absence of DNase I incorporates 10nMol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a template primer
• Unit Reaction Conditions: 50mM potassium phosphate (pH 7.5), 6.7mM MgCl₂ , 1mM 2-mercaptoethanol, 80μg/mL template•primer, 32μM dTTP, 69nM [^{3H]dTTP, and enzyme in 100μL for 30 min. at 37°C}

Cloning, Restriction Enzyme Cloning

Order Info

Shipping Condition: Wet Ice

Specifications

• Content And Storage: DNA Polymerase I/DNase I contains 0.5 units DNA Polymerase I and 0.4 milliunits DNase I per microliter. Store at -20°C.
• Polymerase: DNA Polymerase I
• Quantity: 250 U
• Hot Start: No

• Description: DNA Polymerase
• Exonuclease Activity: 3' - 5', 5' - 3'
• Shipping Condition: Wet Ice

For Research Use Only. Not for use in diagnostic procedures.