Invitrogen™ DNA Polymerase I

DNA polymerase with 5'→3' and 3'→5' exodeoxyribonuclease activities

Supplier:  Invitrogen™ 18010025

Catalog No. 18-010-025

Includes: 1000 units of DNA polymerase in a storage buffer of 50mM potassium phosphate (pH 7.0), 100mM KCl, 1mM DTT, and 50% (v/v) glycerol. It is supplied at 5-10 units/μL.

Description

Performance and quality testing
Endodeoxyribonuclease assay; efficiency of DNase I-dependent nick translation determined.

Unit definition
One unit incorporates 10nMol of total deoxyribonucleotide into acid-precipitable material in 30 min at 37°C using template/primer.

Unit reaction conditions
50mM potassium phosphate (pH 7.0), 6.7mM MgCl₂ , 1mM 2-mercaptoethanol, 80μg/mL template/primer, 32μM dTTP, 69nM [^3H]dTTP, and enzyme in 100μL for 30 min at 37°C. Also incorporates biotinylated nucleotides.

• Applications: DNase I-dependent nick translation, second-strand synthesis in cDNA cloning, fill-in of 5' overhangs
• Source: purified from E. coli expressing the DNA Polymerase I gene on a plasmid

Cloning, Restriction Enzyme Cloning

Order Info

Shipping Condition: Wet Ice

Specifications

• Content And Storage: 1000 units of DNA polymerase in a storage buffer of 50 mM potassium phosphate (pH 7.0), 100 mM KCl, 1 mM DTT, and 50% (v/v) glycerol. It is supplied at 5–10 units/μl.
  
  Store at -5°C to -30°C.
• Polymerase: DNA Polymerase I
• Quantity: 1000 U
• Hot Start: No

• Description: DNA Polymerase
• Exonuclease Activity: 3' - 5', 5' - 3'
• Shipping Condition: Wet Ice

For Research Use Only. Not for use in diagnostic procedures.