Invitrogen™ AccuPrime™ GC-Rich DNA Polymerase

AccuPrime™ GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content.

Supplier:  Invitrogen™ 12337016

Catalog No. 12-337-016

Includes: AccuPrime GC-Rich DNA Polymerase (100μL, at 2U/μL) is supplied with two 5X reaction buffers and a separate vial (1mL) of 50mM MgSO₄ . Both buffers contain dNTPs, MgSO₄ , and AccuPrime accessory proteins. Reaction buffer A (1mL) is for genomic targets. Reaction buffer B (1mL) is for use with cDNA, plasmid, or lambda targets. Sufficient reagents are provided for 200 amplification reactions of 25μL each (at 1 unit of enzyme per reaction).

Description

AccuPrime™ GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content. The kit offers a choice of buffers for amplifying genomic DNA targets (Buffer A) or non-GC-rich cDNA, plasmid, and lambda-based targets (Buffer B).

Benefits of using AccuPrime™ GC-Rich DNA Polymerase:
• Yield—high yields for targets up to 5 kb in length
• Specificity—AccuPrime™ accessory proteins for improved PCR specificity
• Sensitivity—sensitivity down to 5 ng of template DNA

Robust and specific amplifications
The AccuPrime™ buffers contain thermostable proteins that enhance primer-template hybridization during PCR, increasing the specificity of the reaction. The polymerase itself, from the archaebacterium Pyrolobus fumarius, has a five-fold better processivity than Taq DNA polymerase, and remains active even after 4 hours at 95°C.

Unit definition
One unit of AccuPrime™ GC-Rich DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of dNTPs into acid-insoluble material in 30 min at 74°C.

Order Info

Approved for shipment on Wet or Dry Ice

Specifications

• Content And Storage: • AccuPrime GC-Rich DNA Polymerase (1 x 100 μL at 2 U/μL)
  • 5X AccuPrime GC-Rich Buffer A (1 x 1 mL)
  • 5X AccuPrime GC-Rich Buffer B (1 x 1 mL)
  • 50 mM MgSO₄ (1 x 1 mL)
  
  Store at –20°C.
  
  
• Polymerase: AccuPrime GC-Rich DNA Polymerase
• Shipping Condition: Wet or Dry Ice
• Fidelity (vs. Taq): 2X
• No. of Reactions: 200 Reactions
• Reaction Format: Separate Components
• Volume: 100 μL

• GC-Rich PCR Performance: High
• Reaction Speed: Standard
• For Use With (Application): Hot-start PCR
• Hot Start: Built-In Hot Start
• Overhang: Blunt
• Size (Final Product): 5 kb or less

For Research Use Only. Not for use in diagnostic procedures.