Thermo Scientific™ Imject™ Maleimide-Activated mcKLH Spin Kit

Perform hapten-carrier conjugation with cysteine-containing peptides and other thiolated antigens with this sulfhydryl-reactive mariculture KLH.

Supplier:  Thermo Scientific™ 77666

Catalog No. PI77666

Description

Thermo Scientific™ Imject Maleimide-Activated mcKLH is sulfhydryl-reactive immunogenic mariculture keyhole limpet hemocyanin (KLH) for hapten-carrier conjugation with cysteine-containing peptides and other thiolated antigens.

The high-quality mariculture KLH is rendered sulfhydryl-reactive by chemical modification with Sulfo-SMCC crosslinker, and the resulting maleimide-activated protein is then stabilized and lyophilized in phosphate buffer. Maleimide groups form covalent crosslinks with sulfhydryl (-SH) moieties on cysteine residues of peptides and other thiol-containing haptens. Numerous antigen molecules can be conjugated to each KLH molecule to make them highly immunogenic and increase the likelihood of eliciting immunized animals to produce high-titer antisera to the epitopes of interest. Select from among several package sizes of activated KLH protein, or choose the complete conjugation kit that includes optimized buffers and trouble-free spin desalting columns.

Highlights:

• Convenient packages – choose stabilized, lyophilized Maleimide-Activated mcKLH or a complete immunogen conjugation kit

• Optimized activation – specifications require that each lot is activated with more than 400 moles of sulhydryl-reactive maleimide per mole of KLH, ensuring consistently high levels of conjugation

• Sulfhydryl-reactive – engineered for conjugating cysteine-containing peptide antigens to make effective immunogens for high-titer antibody production

• Simple, rapid procedure – complete the hapten-carrier conjugation reaction in two hours to yield sufficient immunogen for several injections and booster immunizations of host animals

• High-quality carrier protein – KLH is isolated from the mollusk Megathura crenulata (keyhole limpet); mcKLH is a high-quality form of KLH that is harvested from limpets grown in mariculture

• Highly immunogenic – KLH has a high molecular mass (0.5 to 13 million daltons; aggregates of 350 and 390kDa subunits) and elicits a stronger immune response than BSA or ovalbumin

• Excellent solubility – our preparations of mariculture KLH are more soluble than typical forms and we also offer a PEGylated form for even greater hydrophilicity and solubility

Includes:

Kit contains stabilized, lyophilized Maleimide-Activated mcKLH (in PBS with stabilizer), buffers and desalting columns to easily prepare ready-to-use immunogens for immunization

Specifications

• Quantity: 5 x 2 mg
• Description: Imject Maleimide Activated mcKLH Spin Kit
• Product Line: Imject™

• Content And Storage: Sufficient For: 5 conjugation reactions and complete immunogen preparations, each with up to 2 mg sulfhydryl-containing peptide
  • Imject Maleimide-Activated mcKLH, 5 x 2 mg
  • Imject Maleimide Conjugation Buffer, 30 mL
  • Imject Purification Buffer Salts, 5 x 0.84 g
  • Zeba Spin Desalting Columns, 7K MWCO, 2 mL, 5 columns
  
  Store the Maleimide-Activated blue Carrier Protein at -20°C. Store remaining components at 4°C.
• Format: Kit

For Research Use Only. Not for use in diagnostic procedures.