Invitrogen™ IL-8 Human Matched Antibody Pair

Matched Antibody Pair

Supplier:  Invitrogen™ BMS2043MST

Catalog No. 50-179-43

Includes: 1 vial (5.5mL) monoclonal Coating Antibody to human IL-8/NAP-1 (100μg/mL)
1 vial (55μL) Biotin-Conjugate anti-human IL-8/NAP-1 polyclonalantibody
1 vial (11μL) Streptavidin-HRP
1 vial (30μL) human IL-8/NAP-1 Standard protein, 200ng/mL
2 vials (50mL) Sample Diluent

Description

The Human IL-8/NAP-1 Matched Antibody Pair Kit comes with pre-matched antibody pairs,standards,and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-8. Buffer reagents and microplates needed to complete the ELISA reaction are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate,which is an overnight process. Samples,standards,or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody,a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

IL-8 (Interleukin-8) is a 72 amino acid pro-inflammatory CXC chemokine designated CXCL8,secreted by endothelial cells and monocytes,which acts as a major chemoattractant for neutrophils and also T cells and basophils. IL-8,first isolated from osteosarcoma cells,contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1),granulocyte chemotactic protein 1 (GCP-1),monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C.

Specifications

• Antigen: IL-8
• Classification: Monoclonal/Polyclonal
• Gene Accession No.: P10145
• Gene Symbols: CXCL8
• Regulatory Status: RUO
• Gene ID (Entrez): 3576
• Content And Storage: -20°C

• Applications: ELISA
• Conjugate: Biotin
• Gene Alias: Protein 3-10C, CXCL8, NAP-1, GCP-1
• Quantity: 10 x 96 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: ELISA Kit

For Research Use Only.