IgG2b Mouse anti-Rat, eFluor™ 570, eBioscience™

Mouse Monoclonal Secondary Antibody

Supplier:  Invitrogen 41481582

Catalog No. 50-112-3625

Description

Description: The monoclonal antibody R2B-7C3 recognizes Rat IgG2b antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other rat isotypes nor does it crossreact to mouse IgG2b or any mouse isotype antibodies. Applications Reported: This R2B-7C3 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry. Applications Tested: This R2B-7C3 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded sections that have been stained first with a rat IgG2b anti-human antibody. This can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filter Recommendation: When using this eFluor™ 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor™ 555 or TRITC filter is acceptable. Excitation: 555 nm; Emission: 570 nm

Secreted immunoglobulins (Ig) have two separable functions: one is to bind specifically to pathogenic molecules, the other is to recruit cells and molecules to destroy the opsonized pathogen.

Specifications

• Antigen: Rat IgG2b
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Secondary Antibody
• Monoclonal or Polyclonal: Monoclonal

• Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
• Clone: R2B-7C3
• Conjugate: eFluor™ 570
• Format: Liquid
• Isotype: IgG1, kappa
• Quantity: 100 μg
• Storage Requirements: 4° C, store in dark, DO NOT FREEZE!
• Primary or Secondary: Secondary
• Target Species: Rat

For Research Use Only.