IFN-γ Mouse, anti-Human, RB780, Clone: B27, BD Horizon™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
BDB568687	BD Biosciences 568687	25 Tests
BDB568686	BD Biosciences 568686	100 Tests

Description

The B27 monoclonal antibody specifically binds to human interferon-γ (IFN-γ), a 14-18 kDa glycoprotein containing 143 amino acid residues. IFN-γ is a potent multifunctional cytokine produced by several activated cell types including NK, NKT, CD4+TCRαβ+, CD8+TCRαβ+, and TCRγδ+ T cells. IFN-γ exerts its biological effects through specific binding to the high-affinity IFN-γ receptor complex comprised of IFN-γRα (CD119) and IFN-γRβ subunits. In addition to its antiviral effects, IFN-γ upregulates a number of lymphoid cell functions including the antimicrobial and anti-tumor responses of macrophages, NK cells, and neutrophils. In addition, IFN-γ influences the regulation of proliferation, differentiation, and effector responses of B cell and T cell subsets. These influences can involve IFN-γ's capacity to boost MHC class I and II expression by antigen-presenting cells as well as direct effects on B cells and T cells themselves. B27 is a neutralizing antibody. The use of B27 antibody for epitope mapping of human IFN-γ has been described. The B27 antibody has been reported not to bind to denatured IFN-γ.

Specifications

• Antigen: IFN-γ
• Applications: Flow Cytometry
• Host Species: Mouse
• Target Species: Human
• Gene ID (Entrez): 3458
• Isotype: IgG1 κ
• Content And Storage: Refrigerate (2°C to 8°C)

• Clone: B27
• Conjugate: RB780
• Regulatory Status: RUO
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide
• Immunogen: Human IFN-γ Recombinant Protein
• Primary or Secondary: Secondary