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Invitrogen™ Silencer™ Select GAPDH Positive Control siRNA

Silencer™ Select GAPDH Positive Control siRNA

Specifications

Content And Storage The siRNA is provided dried down in a single tube, along with Nuclease-free Water for resuspension, and should be stored at –20°C.
Control Type Positive Control
RNAi Type siRNA
For Use With (Application) RNAi, Transfection
Label or Dye Unconjugated
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Description

Description

Ambion™ Silencer™ SelectGAPDH Positive Control siRNA is extensively validated and an ideal control for many aspects of an siRNA experiment. 5 nmol is provided.

  • Premium-quality siRNA, highly purified and ready to use
  • Functionally tested in several common cell lines
  • Include Silencer™ Select modifications for enhanced specificity
  • For use in human, mouse, and rat cells

    What are Silencer™ Select siRNAs?
    Silencer™ Select siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications, to yield siRNAs with unrivalled efficacy, potency, and specificity. The result is fewer failed experiments due to poor silencing, and cleaner, more consistent phenotypic data.

    Silencer™ Select GAPDH Positive Control siRNA:
    The Silencer™ SelectGAPDH positive control is extensively validated and an ideal test siRNA for those just beginning siRNA experiments, because it is validated to work in multiple cell lines. Because it targets GAPDH mRNA (a common internal control), many assays are available to measure its effects, including real-time RT-PCR as well as the Ambion™ KDalert™ GAPDH Assay Kit.

    Quality Control:
    Silencer™ Select siRNAs are manufactured in a state-of-the-art facility under the highest quality standards. Rigorous quality control procedures include MALDI-TOF mass spectrometry analysis and analytical HPLC to monitor purity. Each siRNA is also assessed by gel electrophoresis to confirm that the strands anneal properly.

    Accessory Product: siPORT™ NeoFX™ Transfection Agent (SKU #AM4510 and #AM4511)
    siPORT™ NeoFX™ Transfection Agent is a versatile, lipid-based transfection agent for the efficient transfection of siRNA into cultured cells. The protocol can be adapted to a wide range of cells and experimental designs, including high-throughput applications. Silencer™ Select siRNA controls have specific chemical modifications; they are designed to be used with Silencer™ Select siRNAs. If you are using other siRNA products, such as BLOCK-iT™ siRNAs, Ambion™ Silencer™ siRNAs, or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.
  • Specifications

    Specifications

    The siRNA is provided dried down in a single tube, along with Nuclease-free Water for resuspension, and should be stored at –20°C.
    siRNA
    Unconjugated
    siRNA
    Room Temperature
    Positive Control
    RNAi, Transfection
    Silencer™, Silencer™ Select, Ambion™
    HPLC
    GAPDH
    Videos
    SDS
    Documents

    Documents

    Product Certifications

    For Research Use Only. Not for use in diagnostic procedures.

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