eBioscience™ Human/Non-Human Primate Regulatory T Cell Staining Kit #1

Antibody Staining Kit

Supplier:  Invitrogen 88499940

Catalog No. 50-171-67

Includes:

• Flow Cytometry Staining Buffer (cat. 00-4222): 200mL, store at 2-8°C.
• Fixation/Permeabilization Concentrate (4X) (cat. 00-5123): 30mL, store at 2-8°C. Avoid agitation.
• Fixation/Permeabilization Diluent (cat. 00-5223): 100mL, store at 2-8°C.
• Permeabilization Buffer (10X) (cat. 00-8333): 100mL, store at 2-8°C.
• Normal Rat Serum (cat. 24-5555): 100 μL, store at 2-8°C.
• Anti-Human CD4 FITC (clone OKT4, cat. 11-0048): 25 tests, store at 2-8°C. Light-sensitive material.
• Anti-Human CD25 APC (clone BC96, cat. 17-0259): 25 tests, store at 2-8°C. Light-sensitive material.
• Anti-Human Foxp3 PE (clone PCH101, cat. 12-4776): 25 tests, store at 2-8°C. Light-sensitive material.
• Rat IgG2a K Isotype Control PE (cat. 12-4321): 25 tests, store at 2-8°C. Light-sensitive material.

Description

This Human/Non-human Primate Regulatory T cell Staining Kit contains all of the buffers and monoclonal antibodies for CD4, CD25, and Foxp3 necessary to successfully stain and identify regulatory T cells from human, rhesus, cynomolgus, or chimpanzee peripheral blood cells. This kit may also be used to identify regulatory T cells in baboons, using the CD25 and Foxp3 monoclonal antibodies, however, the monoclonal antibody for CD4 that is included in this kit does not crossreact with baboon.

The OKT4 monoclonal antibody reacts with human, rhesus, cynomolgus, and chimpanzee CD4, a 59 kDa glycoprotein found on the surface of the majority of thymocytes, a subset of mature T cells (T helper cells), and at lower levels on monocytes. The BC96 monoclonal antibody reacts with human, rhesus, cynomolgus, chimpanzee, and baboon CD25 (also known as interleukin-2 receptor alpha, IL-2R alpha), a 55 kDa surface protein expressed by early progenitors of T cells and B cells, by mature, activated T cells and B cells, and at constitutively high levels on regulatory T cells. The PCH101 monoclonal antibody reacts with the amino terminus of human, rhesus, cynomolgus, chimpanzee, and baboon Foxp3, also known as FORKHEAD BOX P3, SCURFIN, and JM2. Foxp3 is a 49-55 kDa protein and a member of the forkhead/winged-helix family of transcription factors. It was identified as the gene responsible for the X-linked lymphoproliferative disease observed in scurfy (sf) mice and in the human disorder, X-linked autoimmunity-allergic dysregulation syndrome (XLAAD). Constitutive expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

Specifications

• Antigen: CD4/CD25/Foxp3
• Classification: Monoclonal
• Conjugate: FITC/APC/PE
• Regulatory Status: RUO
• Target Species: Human, Monkey
• Product Line: eBioscience™

• Applications: Flow Cytometry
• Clone: OKT4, BC96, PCH101
• Quantity: Kit
• Primary or Secondary: Primary
• Content And Storage: Store at 2-8°C. Light sensitive material. Use within 6 months of opening or by date indicated on the bottle.

For Research Use Only. Not for use in diagnostic procedures.