Cytiva HiTrap™ NHS-Activated HP Columns

Couple ligands containing primary amines easily. Cytiva Life Sciences™ HiTrap™ NHS-Activated HP Columns are prepacked with NHS activated Sepharose™ High Performance.

Supplier:  Cytiva 17071601

Catalog No. 45-000-137

Includes: Complete instructions for use and method optimization, plus a variety of connectors for syringe, peristaltic pump, and chromatography systems

Description

Prepacked with NHS-activated Sepharose™ High Performance Media for coupling ligand via primary amines at close to neutral pH

• Designed for use with a syringe, peristaltic pump, or chromatography system
• Degree of substitution: 10 μmol NHS/mL medium
• Delivered in 100% isopropanol to protect the active groups
• Stable in the pH range 3 to 12, depending upon ligand
• Average particle size: 34μm

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Specifications

• Quantity: 5 x 1mL
• Particle Size: 34 μm
• Bed Volume: 1mL
• Diameter (Metric) Inner: 0.7cm
• Height (English): 0.98 in.
• Ligand Type: 10μM NHS groups/mL medium
• Max. Pressure: 42psi
• Support Type: 6% Highly Cross-linked Agarose

• Flow Rate: 4 mL/min.
• For Use With (Equipment): Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
• Product Type: NHS-Activated HP Column
• For Use With (Application): Preparative affinity chromato graphy, easy coupling of ligands containing primary Amines
• Height (Metric): 2.5cm
• Material: Polypropylene
• pH Range: 3 to 12
• Content And Storage: 4° to 8°C