Cytiva HiTrap™ HIC Sepharose™ Selection Kit

Use for laboratory and intermediate process-scale separations and final-step purifications. Cytiva Life Sciences™ HiTrap™ HIC Sepharose™ Selection Kit takes full advantage of hydrophobic interaction chromatography providing highest resolution.

Supplier:  Cytiva 28411007

Catalog No. 45-001-471

Description

• Includes seven 1mL HIC columns, each packed with a different medium for small-scale media screening and selection
• Flexible design for use with syringes, single laboratory pump or chromatography system
• High flowrates and high capacities

Media in Kit Columns:
Phenyl Sepharose High Performance

• For laboratory and intermediate process-scale separations and final-step purifications needing a high resolution

Phenyl Sepharose 6 Fast Flow™ (low sub) and Phenyl Sepharose 6 Fast Flow (high sub)

For initial and intermediate step purifications requiring a matrix with low to medium hydrophobicity

Two available degrees of substitution increases likelihood of finding the optimal selectivity andcapacity for a given application

Butyl Sepharose High Performance

• For intermediate and final purification steps
• Relatively high ligand density enables high dynamic binding capacity for proteins, even at low salt concentrations

Butyl Sepharose 4 Fast Flow

• For initial and intermediate step purifications requiring a matrix with low to medium hydrophobicity
• Often works well even in relatively low salt concentrations

Butyl-S Sepharose 6 Fast Flow

• Least hydrophobic option in the Fast Flow series
• Suited for binding and elution of relatively strong hydrophobic proteins

Octyl Sepharose 4 Fast Flow

• Different hydrophobic character from the phenyl and butyl ligands

Specifications

• Bed Volume: 1 mL
• Diameter (Metric) Inner: 7 mm
• Flow Rate: < 4 mL/min
• Includes: Seven HiTrap 1 mL column containing the following media: Phenyl Sepharose high performance, Butyl Sepharose high performance, Phenyl Sepharose 6 fast flow (low sub), Phenyl Sepharose 6 fast flow (high sub), Butyl-S Sepharose 6 fast flow, Butyl Sepharose 4
• Max. Pressure: 5 bar [0.5 MPa] (70 psi)

• Diameter (English) Inner: 0.275 in.
• Product Type: HiTrap HIC Selection Kit
• Quantity: 7 x 1 mL
• Material: Polypropylene
• Storage Requirements: 4°C to 30°C, 20% Ethanol