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Cytiva HisTrap™ FF Columns: 16 x 25mm
Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.
Supplier: Cytiva 17531901
Alert:
MTO (Made-To-Order) item. This item is subject to the following cancellation policy: This item may be cancelled within 10 days of order placement otherwise is not returnable for credit
Description
- Prepacked with precharged Ni Sepharose™ 6 Fast Flow media
- Column material: polypropylene
- Bed dimensions: 16 × 25mm
- Flow velocity: <20mL/min.
- Protein binding capacity of ∽40mg/mL media with negligible nickel ion leakage
- Compatible with wide range of reducing agents, detergents, denaturants, and other additives
- Simple operation with syringe, perstaltic pump, or chromatographic system
- Columns may be connected in series to increase capacity
Medium
- Matrix: 6% cross-linked agarose
- Average particle size: 90μm
- pH stability working range: 3 to 12
Specifications
| 5 | |
| 16 mm | |
| <20 mL/min. | |
| 3 to 12 | |
| 4°C to 30°C, 20% Ethanol | |
| 5 x 1 mL |
| 0.6 in. | |
| HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). | |
| Polypropylene | |
| 6% Cross-linked Agarose | |
| 5 bar (0.5 MPa, 70 psi) | |
| Pre-packed Column |
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