Cytiva HisPrep Column

Optimize purification of histidine-tagged recombinant proteins. Cytiva Life Sciences™ HisPrep Column is a ready to use column offering convenient scale-up purification of histidine-tagged proteins.

Supplier:  Cytiva 28936551

Catalog No. 45-002-073

Description

• Optimized for convenient scale-up purification of histidine-tagged proteins
• Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
• Negligible leakage of Ni2+
• Bed Volume 20ml
• High binding capacity
• Convenient and time-saving prepacked HiPrep format with precharged Ni Sepharose 6 Fast Flow medium

• Media:
• BioProcess media
• 90μm average particle size
• Metrix 6% cross-linked agarose
• pH stability working range 3-12¹
• Metal ion capacity ∽15μmol Ni2+™/ml medium
• Three years shelf life
• Stable in commonly used aqueous buffers - 0.1 M HCl, 0.1 M NaOH, 8 M urea3

• Column:
• Inside Diameter is 16mm
• Material of Column hardware and tube is Polypropylene and bed support is Polymethyl-Methacrylate
• Bed support Filter

• Bed Dimensions 16 x 100mm
• Flow rate <10ml/min¹
• Flow Velocity <300cm/h
• Pressure Max. 1.5 bar

Specifications

• Diameter (English) Inner Column: 0.6 in.
• Content And Storage: 4°C to 30°C
• Volume (Metric): 20 mL

• Flow Rate: <10 mL/min.
• Diameter (Metric) Inner Column: 1.6 cm
• Quantity: Each