Gibco™ Sf-900™ III SFM

A low-hydrolysate, serum-free, protein-free, animal origin–free insect cell culture medium

Catalog Number	Mfr. No.	Quantity
12-658-019	Gibco™ 12658019	500 mL
12-658-027	Gibco™ 12658027	1000 mL
12-658-035	Gibco™ 12658035	10 L
50-591-352	Gibco™ 12658001	20 L

Description

• Sf-900™ III SFM is a low-hydrolysate, serum-free, protein-free, animal origin–free insect cell culture medium optimized for the growth and maintenance of Spodoptera frugiperda (Sf9 and Sf21) cells
• Also optimized for recombinant gene expression using the baculovirus and stable insect expression systems
• Medium is suitable for suspension and monolayer culture methods and supports growth of other lepidopteran cell lines
• Spodoptera frugiperda (Sf9) cells grown in Sf-900™ III SFM achieve maximum cell densities of 10 to 14 × 10⁶ cells/mL
• Sf-900™ III SFM is an improved serum-free, protein-free, animal origin–free medium designed for growth of Sf9 and other lepidopteran cell lines and production of insect viruses and recombinant proteins
• Ready to use format does not require addition of serum, glutamine, or surfactants
• Cells adapted to other commercially available serum-free media should be sequentially adapted into Sf-900™ III SFM=
• Reduced hydrolysate concentrations improve lot-to-lot consistency
• Store at 2°C to 8°C, protected from light

Order Info

Shipping Condition: Room temperature

Compliance

Gibco Sf-900 II SFM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Specifications

• Cell Line: Sf21, Sf9
• Form: Liquid
• Serum Level: Serum-free
• Product Line: Gibco™, Sf-900™

• Cell Type: Insect Cell
• Product Type: Insect Cell Serum Free Medium (SFM)
• With Additives: Glutamine
• Shipping Condition: Room Temperature

For Research Use Only. Not for use in diagnostic procedures.