Gibco™ RPMI 1640 Medium, GlutaMAX™ Supplement, HEPES

Originally developed to culture human leukemic cells in suspension and as a monolayer

Supplier:  Gibco™ 72400146

Catalog No. 72-400-146

Description

RPMI 1640 Medium (Roswell Park Memorial Institute 1640 medium) has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.

• Modified as follows: with GlutaMAX™, phenol red, HEPES
• Contains reducing agent glutathione and high concentrations of vitamins
• Contains biotin, vitamin B₁₂ and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium
• Vitamins inositol and choline are present in very high concentrations
• GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format
• Contains no proteins, lipids, or growth factors
• Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
• Uses sodium bicarbonate buffer system (2.0g/L), and therefore requires a 5-10% CO₂ environment to maintain physiological pH

Antibody and Cell Culture Production, Bioproduction, Cell Culture, Mammalian Cell Culture

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage: Storage conditions: 2-8° C. Protect from light
  Shipping conditions: Ambient
  Shelf life: 12 months from date of manufacture
• Cell Type: Leukemic Cells
• Concentration: 1 X
• Product Type: RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
• Sterility: Sterile-filtered
• Without Additives: No Sodium Pyruvate
• Quantity: 5 L

• Cell Line: HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
• Classification: Animal Origin-free
• Form: Liquid
• Serum Level: Standard Serum Supplementation
• With Additives: GlutaMAX, HEPES, Phenol Red
• Product Line: Gibco™, GlutaMAX™, SynerGy™
• Shipping Condition: Room Temperature

Safety and Handling

ShelfLife : 12 Months From Date of Manufacture

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.