Gibco™ RPMI 1640 Medium, GlutaMAX™ Supplement

RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) was originally developed to culture human leukemic cells in suspension and as a monolayer.

Catalog Number	Mfr. No.	Quantity
61-870-036	Gibco™ 61870036	500 mL
61-870-127	Gibco™ 61870127	10 x 500 mL

Description

RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI 1640 is modified as follows:
With: GlutaMAX™, Phenol Red
Without: HEPES
The complete formulation is available.

Using RPMI 1640 Medium
RPMI 1640 medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 medium contains biotin, vitamin B₁₂, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 medium with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. RPMI 1640 medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 medium uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage: Storage conditions: 2-8° C. Protect from light
  Shipping conditions: Ambient
  Shelf life: 12 months from date of manufacture
• Cell Type: Leukemic Cells
• Concentration: 1 X
• Product Type: RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
• Sterility: Sterile-filtered
• Without Additives: No HEPES, No Sodium Pyruvate
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Shipping Condition: Room Temperature

• Cell Line: HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
• Classification: Animal Origin-free
• Form: Liquid
• Serum Level: Standard Serum Supplementation
• With Additives: GlutaMAX, Phenol Red
• Green Features: Sustainable packaging
• Product Line: Gibco™, GlutaMAX™

Safety and Handling

ShelfLife : 12 Months From Date of Manufacture

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.