Gibco™ MEM α, nucleosides, no phenol red

Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells.

Catalog Number	Mfr. No.	Quantity
41-061-029	Gibco™ 41061029	500 mL
41-061-037	Gibco™ 41061037	10 x 500 mL

Description

Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of Gibco™ MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM α is modified as follows:
With: Ribonucleosides, Deoxyribonucleosides, L-glutamine
Without: Phenol Red

The complete formulation is available.

Gibco™ MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B₁₂, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR negative cells. This product is made with Earle’s salts.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Compliance

Gibco MEM α is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Specifications

• Content And Storage: Storage conditions: 2-8° C. Protect from light
  Shipping conditions: Ambient
  Shelf life: 12 months from date of manufacture
• Cell Type: Primary Rat Astrocytes
• Concentration: 1 X
• Product Type: MEM α (Minimum Essential Medium α)
• Sterility: Sterile-filtered
• Without Additives: No HEPES, No Phenol Red
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Shipping Condition: Room Temperature

• Cell Line: HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
• Classification: Animal Origin-free
• Form: Liquid
• Serum Level: Standard Serum Supplementation
• With Additives: Low Glucose, Glutamine, Sodium Pyruvate, Ribonucleosides, Deoxyribonucleosides
• Green Features: Sustainable packaging
• Product Line: Gibco™

Safety and Handling

ShelfLife : 12 Months From Date of Manufacture

For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.