Gibco™ MEM α, no nucleosides, powder

Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells.

Catalog Number	Mfr. No.	Quantity
12-000-022	Gibco™ 12000022	10 x 1 L
12-000-063	Gibco™ 12000063	10 L

Description

Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of Gibco™ MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM α is modified as follows:
With: Phenol Red, L-glutamine
Without: Ribonucleosides, Deoxyribonucleosides, Sodium Bicarbonate

The complete formulation is available.

Gibco™ MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B₁₂, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR negative cells. This product is made with Earle’s salts.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH. Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage: Storage conditions: 2-8° C
  Shipping conditions: Ambient
  Shelf life: 36 months from date of manufacture
• Cell Type: Primary Rat Astrocytes
• Form: Powder
• Serum Level: Standard Serum Supplementation
• With Additives: Low Glucose, Glutamine, Phenol Red, Sodium Pyruvate
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Shipping Condition: Room Temperature

• Cell Line: HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
• Classification: Animal Origin-free
• Product Type: MEM α (Minimum Essential Medium α)
• Sterility: Sterile-filtered
• Without Additives: No HEPES, No Ribonucleosides, No Deoxyribonucleosides, No Sodium Bicarbonate
• Product Line: Gibco™

Safety and Handling

ShelfLife : 36 Months From Date of Manufacture

For Research Use Only. Not for use in diagnostic procedures. Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For in vitro diagnostic use.