Gibco™ Medium 199, Earle's Salts, powder

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells.

Supplier:  Gibco™ 31100035

Catalog No. 31-100-035

Description

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco™ Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This M199 is modified as follows:
With: L-glutamine, Phenol Red
Without: HEPES, Sodium Bicarbonate

The complete formulation is available.

Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO₂ incubator, or with Hanks' salts for use without CO₂.

Using Medium 199
Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH. Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage: Storage conditions: 2-8° C
  Shipping conditions: Ambient
  Shelf life: 36 months from date of manufacture
• Cell Type: Chick Embryo Fibroblasts, Mouse Pancreatic Epithelium, Rat Lens Tissues
• Form: Powder
• Sterility: Sterile-filtered
• Without Additives: No HEPES, No Sodium Pyruvate, No Sodium Bicarbonate
• Product Line: Gibco™
• Shipping Condition: Room Temperature

• Cell Line: Rat epithelial
• Classification: Animal Origin-free
• Product Type: Medium 199
• With Additives: Low Glucose, Glutamine, Phenol Red
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Quantity: 10 x 1 L

Safety and Handling

ShelfLife : 36 Months From Date of Manufacture