Gibco™ Ham's F-12 Nutrient Mix

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells.

Catalog Number	Mfr. No.	Quantity
11-765-054	Gibco™ 11765054	500 mL
11-765-062	Gibco™ 11765062	10 x 500 mL
11-765-047	Gibco™ 11765047	1000 mL
11-765-070	Gibco™ 11765070	6 x 1000 mL

Description

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This Ham's F-12 is modified as follows:
With: L-glutamine, Phenol Red

The complete formulation is available.

Using F-12
Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Specifications

• Cell Line: CHO, COS-7, and rat prostate epithelial cells
• Classification: Animal Origin-free
• Form: Liquid
• Sterility: Sterile-filtered
• Without Additives: No HEPES
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard

• Cell Type: Primary Rat Astrocytes
• Concentration: 1 X
• Product Type: Ham's F-12 Nutrient Mixture
• With Additives: Glutamine, Phenol Red, Sodium Pyruvate
• Green Features: Sustainable packaging
• Product Line: Gibco™

Safety and Handling

ShelfLife : 12 Months From Date of Manufacture