Gibco™ Ham's F-10 Nutrient Mix, HEPES

Ham's F-10 Nutrient Mixture was designed for serum-free growth of Chinese hamster ovary (CHO) cells

Supplier:  Gibco™ 12390035

Catalog No. 12-390-035

Description

F-10 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including COS-7, primary rat astrocytes, and rat prostate epithelial cells.

 

• Contains zinc, hypoxanthine, and thymidine
• Serum-free growth of CHO cells in F-10 has led to variety of improved formulations, such as Ham's F-12 Nutrient Mixture (F-12)
• Contains no proteins or growth factors and is modified as follows: with phenol red, HEPES, L-glutamine
• Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
• Uses sodium bicarbonate buffer system (1.2g/L) and therefore requires 5-10% CO₂ environment to maintain physiological pH
• Sustainable packaging

 

Cell Culture, Mammalian Cell Culture

For research use only.

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage: Storage conditions: 2-8° C. Protect from light
  Shipping conditions: Ambient
  Shelf life: 12 months from date of manufacture
• Cell Type: Primary Rat Astrocytes
• Concentration: 1 X
• Product Type: Ham's F-10 Nutrient Mixture
• With Additives: High Glucose, Glutamine, HEPES, Phenol Red, Sodium Pyruvate
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Quantity: 500 mL

• Cell Line: CHO, COS-7, and rat prostate epithelial cells
• Classification: Animal Origin-free
• Form: Liquid
• Sterility: Sterile-filtered
• Green Features: Sustainable packaging
• Product Line: Gibco™
• Shipping Condition: Room Temperature

Safety and Handling

ShelfLife : 12 Months From Date of Manufacture